LeHavreV1, France, Analysis, bibRecord, 001738

Determination of glycols in biological specimens by gas chromatography-mass spectrometry.

Identifieur interne : 001738 ( France/Analysis ); précédent : 001737; suivant : 001739

Determination of glycols in biological specimens by gas chromatography-mass spectrometry.

Auteurs : Vincent Gembus [France] ; Jean-Pierre Goullé ; Christian Lacroix

Source :

Journal of analytical toxicology [ 0146-4760 ]

RBID : pubmed:12166815

English descriptors

KwdEn :
- Calibration, Gas Chromatography-Mass Spectrometry, Glycols (blood), Glycols (poisoning), Humans, Poisoning (diagnosis), Reproducibility of Results, Sensitivity and Specificity.
MESH :
- chemical , blood : Glycols.
- chemical , poisoning : Glycols.
- diagnosis : Poisoning.
- Calibration, Gas Chromatography-Mass Spectrometry, Humans, Reproducibility of Results, Sensitivity and Specificity.

Abstract

A simple extraction and derivatization procedure for the analysis of eight glycols (ethylene glycol, EG; diethylene glycol, DEG; triethylene glycol, TEG; 1,2-propanediol, 1,2-PD; 1,3-propanediol, 1,3-PD; 1,2-butanediol, 1,2-BD; 2,3-butanediol, 2,3-BD; and hexylene glycol, HXG) using a 2-microL serum or blood sample is described. Following deproteinisation with acetonitrile, derivatization to its mono or di TMS derivative, glycols were detected using gas chromatography-electron impact mass spectrometry equipped with a split-spitless inlet and a DB-5MS column in the scan mode from 40 to 500 amu. Gamma-hydroxybutyrate-d6 (GHB-d6) was used as the internal standard. The limits of detection and quantitation in 2 pL of serum ranged, respectively, from 0.7 mg/L for EG to 8.5 mg/L for TEG and from 1.3 mg/L for EG to 18.2 mg/L for 1,2-PD. A linear response was observed over the concentration range from 1 to 800 mg/L for EG and 18 from 800 for TEG and 1,2-PD for serum and blood. Coefficients of variation for both intra-assay precision and interassay reproductibility ranged respectively between 1.9% for TEG to 4.9% for 1,2-PD (11.8% for HXG) and 3.5% for DEG to 9% for 2,3-BD (20.4 for HXG) at the 400 mg/L serum level. The method was applied to plasma and whole blood.

PubMed: 12166815

Affiliations:

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pubmed:12166815

Le document en format XML

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<author><name sortKey="Gembus, Vincent" sort="Gembus, Vincent" uniqKey="Gembus V" first="Vincent" last="Gembus">Vincent Gembus</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Pharmacocinétique et de Toxicologie Cliniques, Groupe Hospitalier du Havre, Le Havre, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Pharmacocinétique et de Toxicologie Cliniques, Groupe Hospitalier du Havre, Le Havre</wicri:regionArea>
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<author><name sortKey="Goulle, Jean Pierre" sort="Goulle, Jean Pierre" uniqKey="Goulle J" first="Jean-Pierre" last="Goullé">Jean-Pierre Goullé</name>
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<author><name sortKey="Lacroix, Christian" sort="Lacroix, Christian" uniqKey="Lacroix C" first="Christian" last="Lacroix">Christian Lacroix</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Determination of glycols in biological specimens by gas chromatography-mass spectrometry.</title>
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<term>Poisoning (diagnosis)</term>
<term>Reproducibility of Results</term>
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<front><div type="abstract" xml:lang="en">A simple extraction and derivatization procedure for the analysis of eight glycols (ethylene glycol, EG; diethylene glycol, DEG; triethylene glycol, TEG; 1,2-propanediol, 1,2-PD; 1,3-propanediol, 1,3-PD; 1,2-butanediol, 1,2-BD; 2,3-butanediol, 2,3-BD; and hexylene glycol, HXG) using a 2-microL serum or blood sample is described. Following deproteinisation with acetonitrile, derivatization to its mono or di TMS derivative, glycols were detected using gas chromatography-electron impact mass spectrometry equipped with a split-spitless inlet and a DB-5MS column in the scan mode from 40 to 500 amu. Gamma-hydroxybutyrate-d6 (GHB-d6) was used as the internal standard. The limits of detection and quantitation in 2 pL of serum ranged, respectively, from 0.7 mg/L for EG to 8.5 mg/L for TEG and from 1.3 mg/L for EG to 18.2 mg/L for 1,2-PD. A linear response was observed over the concentration range from 1 to 800 mg/L for EG and 18 from 800 for TEG and 1,2-PD for serum and blood. Coefficients of variation for both intra-assay precision and interassay reproductibility ranged respectively between 1.9% for TEG to 4.9% for 1,2-PD (11.8% for HXG) and 3.5% for DEG to 9% for 2,3-BD (20.4 for HXG) at the 400 mg/L serum level. The method was applied to plasma and whole blood.</div>
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Determination of glycols in biological specimens by gas chromatography-mass spectrometry.

Determination of glycols in biological specimens by gas chromatography-mass spectrometry.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur la visibilité du Havre
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